Immunofluorescent images of dorsal root ganglion. A. Transient receptor potential vanilloid type 1 (TRPV1) immunoreactive (IR) neurons and merged images with neurofilament (NF) 200 and c–Fos observed through a confocal microscope. Immunofluorescent images showing neurons positive for TRPV1 (red), NF200 (green) and c–Fos (blue) in the dorsal root ganglion (DRG) 9 days after surgery in a) sham operated, b) spinal nerve ligation (SNL), and c) 0.1 mg diluted bee venom (DBV) treated SNL rats. Clockwise from upper left: c–Fos– NF200 –merged – TRPV1. B. Transient receptor potential ankyrin type 1 (TRPA1) IR neurons and merged images with NF200 and c–Fos. Neurons positive for TRPA1 (red), NF200 (green) and c–Fos (blue) in the DRG 9 days after surgery in a) sham operated, b) SNL, and c) 0.1 mg DBV treated SNL rats. Clockwise from upper left: c–Fos– NF200 –merged – TRPA1. C. Transient receptor potential melastatin type 8 (TRPM8) IR neurons and merged images with NF200 and c–Fos observed through a confocal microscope. Neurons positive for TRPM8 (red), NF200 (green) and c–Fos (blue) in the DRG 9 days after surgery in a) sham operated, b) SNL, and c) 0.1 mg DBV treated SNL rats. Clockwise from upper left: c–Fos– NF200 –merged – TRPA1. All images are magnified × 630, with scale bars = 40 μm. Three rats in each group were analyzed.