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. Author manuscript; available in PMC: 2014 Nov 28.
Published in final edited form as: Am J Vet Res. 2010 Jan;71(1):103–114. doi: 10.2460/ajvr.71.1.103

Figure 2.

Figure 2

Photomicrographs of SDFTNs (A; light microscopy; bar = 20 μm) seen as GFP-positive cells via fluorescent microscopy (B; bar = 20 μm). Quantitative assessment of transduction efficiency via flow cytometry analysis; representative scatterplots of control SDFTNs (C) and SDFTN-GFP cells (D) are illustrated. Notice > 95% transduction efficiency in SDFTN-GFP samples. P2 = Region of cells lacking detectable fluorescent activity on the FITC-A channel. P3 = Region of detectable fluorescent activity in analyzed cells. FITC-A = Designated laser used to detect fluorescent activity. Y-axis = Channel numbers detected by use of the indicated laser. X-axis = Number of cells analyzed.