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. Author manuscript; available in PMC: 2014 Nov 28.
Published in final edited form as: J Invest Dermatol. 2009 Nov 19;130(4):968–978. doi: 10.1038/jid.2009.357

Figure 3. Immunohistochemical staining of the epidermis and oral epithelium using a panel of desmoplakin (DP)-specific antibodies (Abs).

Figure 3

(a) Schematic diagram of DP depicts regions of antibody recognition. (b) All antibodies showed cell surface staining throughout the normal adult epidermis (upper panel). In patient's skin, the multiclonal epitope antibody (DP mix) shows significant but disrupted staining of DP in the skin, whereas the C-terminal antibody 10F6 does not detect appreciable levels of DP. Two different antibodies (2C8 and 2A8) detecting parts of the C-terminal domain of DP show clear reduction in patient's oral mucosa compared with normal oral epithelium. Typical cell-cell border staining of desmoglein 1 (Dsg1) and plakoglobin (PG) (c) is observed in both control and patient's skin. Staining for β-catenin in formalin-fixed, paraffin-embedded patient's skin is reduced when compared with that of control epidermis (possibly because of formalin fixation). However, β-catenin expression does not seem to be altered in patient's oral mucosa when compared with that of the control section. Bar=50μm.