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. 2014 Aug 28;2(8):e12121. doi: 10.14814/phy2.12121

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Diagnoses of ischemia and necrosis in pigs with occluded LCx. (A) ECG traces were recorded in each animal before LCx occlusion and used as basal traces (B) ECG traces showed important unlevelling of ST segment after occlusion suggesting beginning of ischemia. (C) Ninety‐six percent of the animals showed ST alternation between 2 and 10 min after LCx occlusion. To confirm the necrosis injury two animals had their blood sampled for about 10 h after LCx occlusion. (D) Ultrasensitive Troponin T dosage showed significant increase above reference value for MI 3 h after occlusion (ANOVA, *P < 0.05, time 0 vs other times, n = 4) (E) and CKMB showed a trend to be higher than reference value 6 h after occlusion (n = 2) corroborating Troponin T data.