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. 2014 Aug 8;2(8):e12092. doi: 10.14814/phy2.12092

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Location of NTS microinjection site. (A) Coronal section of the brainstem of a rat showing the sites of bilateral microinjections of Evan's blue, which is within the 500 μm target area of the caudal NTS where carotid body afferents have a primary synapse and a ventilatory response to glutamate microinjection is evoked. Magnification 6.3×. (B) Sample time course of ventilatory responses to 50 nL glutamate microinjections in two different rats before and after cocktail microinjection. Animals were not used for further measurements or analysis if they showed no response to glutamate or if the cocktail did not block the effects of glutamate.