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. 2014 Aug 13;2(8):e12107. doi: 10.14814/phy2.12107

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — FLCN primarily localizes to the cytoplasm and does not regulate proliferation of lung‐derived cells. (A) Nuclear and cytoplasmic fractions were prepared from SAEC, HEK293, and HeLa cells. FLCN localized primarily to the cytoplasm of all three cell types. CREB was used as a control for the nuclear fraction and GAPDH as a control for the cytoplasmic fraction. Equal amounts of protein in each fraction were loaded. Each panel represents a separate gel. (B) FLCN was downregulated in HBE and SAEC cells using shRNA. Downregulation of FLCN was confirmed by immunoblot. (C) No obvious change in cellular morphology was observed. (D) No difference in cell doubling was observed over 96 h between HBE and SAEC cells expressing control shRNA versus FLCN shRNA.