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FLCN downregulation increases cell–cell adhesion in HBE cells. (A) HBE and SAEC cells with FLCN downregulation or control shRNA were grown as clusters in adherence‐free conditions for 96 h and then sheared using a standardized protocol (see Methods). (B) Immunoblot was used to compare levels of cell adhesion proteins in SAEC and HBE cells with control shRNA or FLCN shRNA cells grown in adherent monolayers or in detached conditions.
