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. 2014 Aug 19;2(8):e12111. doi: 10.14814/phy2.12111

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Electrophysiological recording of inspiratory rhythms in sandwich slices. (A) Central photograph shows the ventral brainstem prior to sectioning. Photographs in the left and right panels show consecutive “sandwich” slices exposed at the appropriate surface to obtain electrophysiological field recordings from the preBötC and XII. (B) Rostrocaudal borders of rhythmic and nonrhythmic sandwich slices. Abscissa at bottom shows distance relative to VII_c. Thirteen slice pairs are illustrated: cyan represents respiratory active slices, whereas magenta indicates quiescent slices. Cyan‐to‐magenta blended bars reflect slices that started out rhythmically active but progressively ceased rhythmic activity over 30–70 min. (C) Mean (±SD) borders of rhythmic and arrhythmic preBötC field recordings are shown with corresponding mean (±SD) borders for slices with active or silent XII field recordings.