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A fluorescent activity band was observed in the ethanol-precipitated culture supernatant of three batches of W. anomalus , indicating the presence of ß-1,3-glucanase activity (Lane 1 to 3). ß-1,3-glucanase activity was detected by overlaying the native gel with 0.2% (wt/vol) MUG in acetate buffer (pH 5) and incubating for 45 min at 30°C before UV illumination.
