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Optimization of DNA concentration for polyethylenimine-mediated LvV production in Opti-MEM®. LvVs were produced using 15×106 HEK 293T cells per 10-cm plate and a total amount of 4.55 μg, 9.1 μg and 13.65 μg plasmid DNA mixture was added to 150 mm NaCl to a final volume of 380 μl, respectively. LvV, lentiviral vector; TU, transduction units.
