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. 2014 Oct 15;9(1):365–370. doi: 10.3892/ol.2014.2621

Figure 2.

Figure 2

mRNA expression of IE1–72, TLR2, TLR4, NF-κB and TNF-α at various time points after SW480 cells were infected. (A) mRNA expression of IE1–72 increased in a time-dependent manner. (B) TLR2 mRNA levels were upregulated at 6 h, exhibited a prominent increase at 12 h, reached their peak at 48 h and decreased gradually after 48 h. (C)No significant differences in TLR4 mRNA were identified. (D) NF-κB mRNA was expressed at high levels, and no significant difference in expression was identified over time. (E) TNF-α mRNA began to increase at 6 h, peaked at 48 h, and decreased gradually after 48 h. (F) The internal reference gene, β-actin. 1, prior to HCMV infection (0 h); 2, 6 h after infection; 3, 12 h after infection; 4,24 h after infection; 5, 48 h after infection; and 6, 72 h after infection; M: marker. TLR, Toll-like receptor; NF, nuclear factor; TNF, tumor necrosis factor; HMCV, human cytomegalovirus.