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. 2014 Oct 24;9(1):47–54. doi: 10.3892/ol.2014.2639

Figure 1.

Figure 1

EPICS® XL™ flow cytometry revealed (A) a high CD133+ expression rate in the CD133+-sorted population and (B) a low CD133+ expression rate in the single-cell suspensions of primary lung cancer cells (89.15±7.80% vs. 2.07±0.21%; P<0.01). Each experiment was repeated three times. Fluorescence microscopy of immunostaining assays (C) detected extensive expression of CD133 in the sphere samples; however, (D) lower levels of CD133 expression were detected in the primary lung cancer cells (staining, DAPI). Green staining presents positive cells. CD, cluster of differentiation.