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. 2014 Nov 28;5:673. doi: 10.3389/fpls.2014.00673

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Copyright © 2014 Nakasu, Edwards, Fitches, Gatehouse and Gatehouse.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Hv1a/GNA on M. persicae via artificial diet. (A) Survival analysis of M. persicae fed on artificial diet alone or containing either GNA or Hv1a/GNA (n = 70 aphids/treatment). (B) Western blot analysis of different samples taken from M. persicae fed with Hv1a/GNA. (1) GNA 100 ng; (2) Hv1a/GNA 100 ng; (3) Aphid diet (negative control); (4) Aphid diet + Hv1a/GNA (0.5 mg/ml); (5) Adult aphids (negative control); (6) Adult aphids after feeding for 24 h with Hv1a/GNA; (7) Adult aphids chase experiment; (8) Aphid nymphs (negative control); (9) Aphid nymphs from adults fed with Hv1a/GNA; (10) Honeydew (negative control); (11) Honeydew from aphids feeding on 0.5 mg/ml Hv1a/GNA. Arrow shows migrating pattern of intact Hv1a/GNA.