Table 3.
Robust minimum 1C-value estimates for several widely different groups of free-living, multicellular, higher organisms obtained by genome sequencing (*), other best practice techniques, or static cytometry using the fluorochrome DAPI for algae1
| Group |
Species |
Mb |
Original reference |
|||
|---|---|---|---|---|---|---|
| ANIMALS | ||||||
| Nematode | Caenorhabditis elegans | 100* | C. elegans sequencing Consortium (1998) | |||
| Platyhelminthes (flatworms) | Stenostomum brevipharyngium | 59 | Gregory et al. (2000) | |||
| Crustacea | Scapholeberis kingii (water flea) | 157 | Beaton (1988) | |||
| Annelid | Dinophilus gyrociliatus (polychaete worm) | 59 | Soldi et al. (1994) | |||
| Tardigrades (water bears) | Isohypsibius lunulatus | 78 | Redi and Garagna (1987) | |||
| Insect | Peristenus stygicus | 98 | TR Gregory (pers. comm.) | |||
| Arachnid | Tetranychus urticae (spider mite) | 78 | TR Gregory (pers. comm.) | |||
| Urochordates (tunicates) | Oikopleura dioica | 72 | Seo et al. (2001) | |||
| PLANTS | ||||||
| Chlorophyta (green alga) | Caulerpa paspaloides | 88 | Kapraun (2005) | |||
| Rhodophyta (red algae) | Heydrichia wolkerlingii | 69 | Kapraun (2005) | |||
| Phaeophyta (brown algae) | Stilophora rhizodes | 98 | Kapraun (2005) | |||
| Bryophyte | Holomitrium arboreum | 167 | Voglmayr (2000) | |||
| Lycophyte | Selaginella kraussiana | 157 | Obermayer et al. (2002) | |||
| Angiosperm | Arabidopsis thaliana | 157 | Bennett et al. (2003) | |||
The use of the base-specific fluorochrome DAPI for estimating DNA amounts may be less reliable than using intercalating fluorochromes such as propidium iodide (e.g. Doležel et al., 1992).