Table 1.

Performance of species with a range of nuclear DNA contents (1C = 0·2–32·35 pg) with the standard AFLP protocol

Species	Ploidy	Ch. no.1	1C-value (pg)2	Genome size3 (pg)	No. of bands scored	No. of bands per sample	No. of polymorphic bands (%)	Primer combination4	No. of samples
Bixa orellana	2 x	14	0·205	0·20	40	33–38	10 (25·0)	E-AAG + M-CAA	4
Zostera marina	2 x	12	0·326	0·32	44	27–33	22 (50·0)	E-AAC + M-CAG	5
Sorbus aria	2 x	34	∼0·657	0·658	50	36–40	25 (50·0)	E-ACT + M-CAC	6
Bromus interruptus	4 x	28	8·439	4·22	92	67–84	35 (38·0)	E-ACT + M-CAG	15
Cephalanthera longifolia	2 x	32 (34)	16·8010	16·80	51	50–51	1 (2·0)	E-ACG + M-CTG	6
Damasonium alisma	6 x	42	23·6211	7·87	26	23–26	3 (11·5)	E-ACT + M-CAG	3
Tulipa sprengeri	2 x	24	∼24·0012	24·00	10	9–10	1 (10·0)	E-ACA + M-CAG	5
Cypripedium calceolus	2 x	20	32·3513	32·35	14	13–14	1 (7·1)	E-AAC + M-CAA	7

¹Chromosome number.

²The DNA amount in the unreplicated gametic nucleus of an organism is referred to as its C-value, irrespective of the ploidy level of the taxon.

³The average DNA amount per genome i.e. 1C-value divided by two for tetraploids and by three for hexaploids. For diploids, this is equivalent to the 1C-value.

⁴The first primer is based on EcoRI (E) and the second on MseI (M). Each has three selective bases as indicated after the hyphen.

⁵Hanson et al., 2001.

⁶Hanson et al., 2003.

⁷This value is approximate as it is that given for the two diploid Sorbus species for which the C-value is known (Bennett and Leitch, 2003).

⁸The whole subfamily Maloideae is thought to have a tetraploid origin (Dickson et al., 1992). If this is the case the genome size is 0·33 pg.

⁹Bennett and Smith, 1976.

¹⁰Bennett and Leitch, 2003.

¹¹L. Hanson, Royal Botanic Gardens, Kew, unpubl. res.

¹²This value is approximate as it is the mean value for diploid Tulipa spp. for which 1C-values are known (Bennett and Leitch, 2003).

¹³Bennett and Leitch, 2003.