Table 3.
The effect of increasing the number of selective bases from six to seven on AFLP in Damasonium alisma
| Species |
1C-value (pg) |
No. of bands scored |
No. of bands per sample |
No. of polymorphic bands (%) |
Primer combination |
No. of samples |
|---|---|---|---|---|---|---|
| D. alisma | 23·62 | 26 | 23–26 | 3 (11·5) | E-ACT + M-CAG | 3 |
| D. alisma | 23·62 | 67 | 65 | 4 (6·0) | E-ACT + M-CAG + A | 2 |
| D. alisma | 23·62 | 54 | 48–53 | 7 (13·0) | E-ACT + M-CAG + C | 2 |
| D. alisma | 23·62 | 41 | 36–42 | 6 (14·6) | E-ACT + M-CAG + G | 2 |
| D. alisma | 23·62 | 49 | 44–47 | 7 (14·3) | E-ACT + M-CAG + T | 2 |
For the large genome primer combinations, the average number of bands scored was 53. The overall percentage polymorphism for the large genome primer combinations was 12·0 %.