Table 2.
Bamboo species-specific SCAR primer sequences derived from cloned RAPD fragments and optimal annealing temperature for each set of reactions
RAPD primer |
SCAR primer* |
Sequence (5′-3′)† |
Annealing temperature |
---|---|---|---|
PW-02 | Balco800F | TCGTCGGCGTAGACGGAGAG | 60 °C |
Balco800R | TCGTCGGCGTTCGAGCTTAT | ||
OPA-08 | Tuldo600F | GTGACGTAGGCGAACATGGC | 60 °C |
Tuldo600R | GTGACGTAGGGCATACCTTG |
The numbers preceding the R (reverse) and F (forward) refer to the approximate size of the SCAR band (in bp), ‘Balco’ and ‘Tuldo’ indicate the two source genomes, B. balcooa and B. tulda, respectively.
The nucleotides underlined represent the sequence of the RAPD primers used.