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. 2005 Feb 24;95(5):835–841. doi: 10.1093/aob/mci088

Table 2.

Bamboo species-specific SCAR primer sequences derived from cloned RAPD fragments and optimal annealing temperature for each set of reactions

RAPD primer
SCAR primer*
Sequence (5′-3′)
Annealing temperature
PW-02 Balco800F TCGTCGGCGTAGACGGAGAG 60 °C
Balco800R TCGTCGGCGTTCGAGCTTAT
OPA-08 Tuldo600F GTGACGTAGGCGAACATGGC 60 °C
Tuldo600R GTGACGTAGGGCATACCTTG
*

The numbers preceding the R (reverse) and F (forward) refer to the approximate size of the SCAR band (in bp), ‘Balco’ and ‘Tuldo’ indicate the two source genomes, B. balcooa and B. tulda, respectively.

The nucleotides underlined represent the sequence of the RAPD primers used.