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. 2014 Nov 7;9(1):143–148. doi: 10.3892/ol.2014.2687

Figure 3.

Figure 3

Activation of the PI3K/Akt downstream pathway was necessary for IGF-I-induced epithelial-to-mesenchymal transition. The serum-starved BGC-823 cells were pretreated with or without (A) IGF-IR inhibitor, OSI-906 (10 μM) or (B) PI3K/Akt inhibitor, LY294002 (100 μM) for 2 h, followed by IGF-I (100 ng/mL) stimulation for 48 h. Cell lysates were collected for western blot analysis. PI3K, phosphoinositide 3-kinase; IGF-I, insulin-like growth factor-I; E-cad, E-cadherin; OSI, OSI-906; LY, LY294002.