Table 3.
Characteristics of fragment variation generated by nine primer combinations in the AFLP analysis of E. angustifolia (n = 30 individuals) and E. sanguinea (n = 3 individuals)
|
E. angustifolia + E. sanguinea |
|||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total |
Mean |
||||||||||||||||||||||||||
| Primer combinations | EM14 | EM15 | EM19 | EM20 | EM22 | EM25 | PM 17 | PM22 | PM25 | EM | PM | Total | EM | PM | |||||||||||||
| Total fragments | 165 | 162 | 166 | 159 | 135 | 166 | 127 | 106 | 104 | 953 | 337 | 1290 | 159 | 112 | |||||||||||||
| Average no. of fragments per plant | 69 | 65 | 65 | 70 | 64 | 75 | 34 | 35 | 26 | 408 | 95 | 503 | 68 | 32 | |||||||||||||
| No. of fragment shared by all populations | 14 | 8 | 6 | 16 | 15 | 15 | 3 | 4 | 0 | 74 | 7 | 81 | 12 | 2 | |||||||||||||
| No. of fragments unique to one population | 15 | 19 | 23 | 15 | 13 | 12 | 25 | 27 | 28 | 97 | 80 | 177 | 16 | 27 | |||||||||||||
| No. of fragments present in two or more populations | 136 | 135 | 137 | 128 | 107 | 139 | 99 | 75 | 76 | 782 | 250 | 1032 | 130 | 83 | |||||||||||||
| Polymorphic fragments (%) | 82·4 | 83·3 | 82·5 | 80·5 | 79·3 | 83·7 | 78·0 | 70·8 | 73·1 | 82 | 74 | 80 | 82 | 74 | |||||||||||||
Fragments were scored within a range of 60–400 bp.