Table 2.
Primers for ISSR analysis
| Primer |
Primer sequence* |
Band size range (bp) |
Tm (°C) |
Total no. of loci |
Total no. of polymorphic loci (%) |
Total no. of distinct phenotypes |
|---|---|---|---|---|---|---|
| ISSR-4 | (AC)8YT | 190–1000 | 44 | 22 | 19 (86·4) | 160 |
| ISSR-6 | (CA)6RY | 180–1200 | 44 | 18 | 15 (83·3) | 124 |
| ISSR-7 | (CA)6RG | 180–2300 | 43 | 36 | 30 (83·3) | 179 |
| ISSR-9 | (CTC)4RC | 210–2150 | 48 | 32 | 24 (75·0) | 169 |
| ISSR-11 | BDB(ACA)5 | 185–1050 | 49 | 24 | 21 (87·5) | 146 |
| ISSR-12 | BBB(GAAA)3GAA | 165–1800 | 43 | 33 | 27 (81·8) | 179 |
| ISSR-13 | (AC)8YG | 190–1700 | 53 | 30 | 27 (90·0) | 178 |
| ISSR-14 | (CAC)4RC | 180–1000 | 60 | 24 | 21 (87·5) | 171 |
*
Y = G/C; R = A/T; B = C/G/T; D = A/G/T.