Table 1.

Names and voucher specimens of Petunia sensu Jussieu taxa used in the cpDNA RFLP analysis

Taxa	Botanical name, collection locality, voucher specimen number and herbaria code*
Petunia sensuWijsman (1990), 2n = 2x = 14
1	P. altiplana T.Ando & Hashim. BRAZIL: Rio Grande do Sul, Ando et al. B319 (S, US)
2	P. axillaris (Lam.) Britton, Sterns & Poggenb. subsp. axillaris. URUGUAY: Montevideo, Ando & Kokubun U1 (MVFA, Ando)
3	P. axillaris subsp. parodii (Steere) Cabrera. URUGUAY: Artigas, Ando & Kokubun U27 (MVFA, Ando)
4	P. axillaris subsp. subandina T.Ando. ARGENTINA: Salta, Ando & Iida A100 (S, SI, Ando)
5	P. bajeensis T.Ando & Hashim. BRAZIL: Rio Grande do Sul, Hashimoto et al. B662 (BM, MBM, MVFA, R, S, SI, SP, U, US, GHSP, Ando)
6	P. bonjardinensis T.Ando & Hashim. BRAZIL: Rio Grande do Sul, Ando et al. B174 (S, GHSP, Ando)
7	P. exserta Stehmann BRAZIL: Rio Grande do Sul, Hashimoto et al. B931 (GHSP)
8	P. guarapuavensis T.Ando & Hashim. BRAZIL: Paraná, Hashimoto et al. B65 (MBM, BM, HBR, S, US, GHSP, Ando)
9	P. inflata R.E.Fr. ARGENTINA: Misiones, Ando & Buto A7 (SI, GHSP, Ando)
10	P. integrifolia (Hook.) Schinz & Thell. subsp. integrifolia. URUGUAY: Río Negro, Ando & Watanabe U106 (MVFA, S, SI, GHSP, Ando)
11	P. integrifolia subsp. depauperata R.E.Fr. BRAZIL: Rio Grande do Sul, Hashimoto et al. B59 (MVFA, SI, GHSP, Ando)
12	P. interior T.Ando & Hashim. BRAZIL: Santa Catarina, Hashimoto et al. B569 (MBM, BM, R, S, US, GHSP, Ando)
13	P. littoralis L.B.Sm. & Downs. BRAZIL: Santa Catarina, Hashimoto et al. B29 (MVFA, GHSP, Ando)
14	P. mantiqueirensis T.Ando & Hashim. BRAZIL: Minas Gerais, Hashimoto et al. B357 (S, BM, K, SP, U, US, GHSP, Ando)
15	P. occidentalis R.E.Fr. ARGENTINA: Jujuy, Ando & Iida A109 (MVFA, S, SI, GHSP, Ando)
16	P. reitzii L.B.Sm. & Downs. BRAZIL: Santa Catarina, Hashimoto et al. B28 (GHSP, Ando)
17	P. riograndensis T.Ando & Hashim. BRAZIL: Rio Grande do Sul, Hashimoto et al. B860 (MBM, S, GHSP, Ando)
18	P. saxicola L.B.Sm. & Downs. BRAZIL: Santa Catarina, Hashimoto et al. B113 (GHSP, Ando)
19	P. scheideana L.B.Sm. & Downs. BRAZIL: Santa Catarina, Hashimoto et al. B109 (BM, MBM, MVFA, S, SI, US, GHSP, Ando)
20	Unnamed taxon P1. BRAZIL: Rio Grande do Sul, Hashimoto et al. B990 (GHSP, Ando)
Petunia sensuJussieu (1803) with 2n = 2x = 18 chromosomes sharing similar morphological characters with Calibrachoa
21	P. alpicola L.B.Sm. & Downs. BRAZIL: Santa Catarina, Hashimoto et al. B130 (BM, MBM, MVFA, S, SI, US, GHSP, Ando)
22	P. helianthemoides Sendtn. ARGENTINA: Misiones, Ando & Buto A4 (SI, Ando)
23	P. kleinii L.B.Sm. & Downs. BRAZIL: Santa Catarina, Hashimoto et al. B105 (MBM, GHSP, Ando)
24	P. pubescens (Spreng.) R.E.Fr. URUGUAY: Durazno, Ando & Kokubun U23 (S, MVFA, Ando)
25	P. variabilis R.E.Fr. URUGUAY: Tacuarembó, Ando & Kokubun U6 (MVFA, Ando)
Calibrachoa La Llave & Lexarza (1825), 2n = 2x = 18
26	C. calycina (Sendtn.) Wijsman. ARGENTINA: Corrientes, Ando & Buto A15 (MBM, GHSP, Ando)
27	C. dusenii (R.E.Fr.) Stehmann & Semir. BRAZIL: Paraná, Hashimoto et al. B484 (MBM, S, GHSP, Ando)
28	C. eglandulata Stehmann & Semir. BRAZIL: Santa Catarina, Hashimoto et al. B917 (GHSP, Ando)
29	C. elegans (Miers) Stehmann & Semir. BRAZIL: Minas Gerais, Hashimoto et al. B489 (GHSP, Ando)
30	C. ericaefolia (R.E.Fr.) Wijsman. BRAZIL: Paraná, Hashimoto et al. B63 (MBM, GHSP, Ando)
31	C. heterophylla (Sendtn.) Wijsman. BRAZIL: Rio Grande do Sul, Hashimoto et al. B60 (BM, MBM, GHSP, Ando)
32	C. linearis (Hook.) Wijsman. URUGUAY: Río Negro, Ando & Buto U165 (MVFA, Ando)
33	C. linoides (Sendtn.) Wijsman. BRAZIL: Rio Grande do Sul, Hashimoto et al. B282 (GHSP, Ando)
34	C. macrodactylon (L.B.Sm. & Downs) Wijsman. BRAZIL: Santa Catarina, Hashimoto et al. B138 (Ando)
35	C. micrantha (R.E.Fr.) Stehmann & Semir. BRAZIL: Paraná, Hashimoto et al. B486 (BM, MBM, S, US, GHSP, Ando)
36	C. parviflora (Juss.) Wijsman. URUGUAY: Tacuarembó, Ando & Kokubun U42 (MVFA, Ando)
37	C. pygmaea (R.E.Fr.) Wijsman. URUGUAY: Artigas, Ando & Kokubun U39 (BM, MVFA, S, US, Ando)
38	C. rupestris (Dusén) Wijsman. BRAZIL: Paraná, Hashimoto et al. B479 (BM, MBM, GHSP, Ando)
39	C. selloviana (Sendtn.) Wijsman. BRAZIL: Rio Grande do Sul, Hashimoto et al. B892 (MBM, S, US, GHSP, Ando)
40	C. sendtneriana (R.E.Fr.) Stehmann & Semir. BRAZIL: Santa Catarina, Hashimoto et al. B447 (BM, US, GHSP, Ando)
41	C. serrulata (L.B.Sm. & Downs) Stehmann & Semir. BRAZIL: Santa Catarina, Hashimoto et al. B1031 (BM, S, US, U, GHSP, Ando)
42	C. spathulata (L.B.Sm. & Downs) Stehmann & Semir. BRAZIL: Santa Catarina, Hashimoto et al. B90 (GHSP, Ando)
43	C. thymifolia (A.St.-Hil.) Stehmann & Semir. BRAZIL: Santa Catarina, Hashimoto et al. B36 (BM, MBM, MVFA, S, SI, US, GHSP, Ando)
44	unnamed taxon C1. BRAZIL: Rio Grande do Sul, Hashimoto et al. B7 (GHSP, Ando)
45	unnamed taxon C2. BRAZIL: Rio Grande do Sul, Hashimoto et al. B201 (GHSP, Ando)
46	unnamed taxon C3. BRAZIL: Santa Catarina, Hashimoto et al. B215 (GHSP, Ando)
47	unnamed taxon C4. BRAZIL: Rio Grande do Sul, Hashimoto et al. B755 (GHSP, Ando)
48	unnamed taxon C5. BRAZIL: Rio Grande do Sul, Hashimoto et al. B789 (GHSP, Ando)
49	unnamed taxon C6. BRAZIL: Santa Catarina, Hashimoto et al. B1126 (GHSP, Ando)
50	unnamed taxon C7. BRAZIL: Rio Grande do Sul, Hashimoto et al. B1176 (GHSP, Ando)
51	unnamed taxon C8. BRAZIL: Santa Catarina, Hashimoto et al. B1248 (GHSP, Ando)
52	unnamed taxon C9. URUGUAY: Salto, Ando & Iida U229 (GHSP, Ando)
Nicotiana L. (1735) 2n = 18, 20, 24, 32, 36, 38, 40, 42, 44, 46, 48
55	Nicotiana langsdorffii Weinm. (2n = 2x = 18). BRAZIL: Rio Grande do Sul, Hashimoto et al. HB622 (Ando)

^*Abbreviations of herbaria are after Holmgren et al. (1990) except GHSP (Centro de Pesquisas História Natural, São Paulo, Brazil) and Ando (temporary collection of Toshio Ando).