Table 2.

ISSR primers used in this study and summary of ISSR markers from 30 Leucadendron cultivars

Primer (UBC)	Primer sequence*	Annealing temperature (°C)†	Fragment size range	Fraction polymorphic fragments‡
813	(CT)8T	50	430–1625	12/12
814	(CT)8A	50	385–1980	19/19
815	(CT)8G	52	395–1690	27/27
817	(CA)8A	52	595–1355	14/15
820	(GT)8T	52	575–2055	16/16
824	(TC)8G	50	595–2230	24/24
826	(AC)8C	52	500–1735	23/25
834	(AG)8YT	52	370–1490	34/34
836	(AG)8YA	52	325–1990	30/31
840	(GA)8YT	54	255–1645	35/35
843	(CT)8RA	54	350–1965	21/21
845	(CT)8RG	54	305–2100	18/18
848	(CA)8RG	55	450–1665	18/19
852	(CT)8RA	52	310–2195	17/18
855	(AC)8YT	55	310–1825	27/28
856	(AC)8YA	55	480–1710	14/14
857	(AC)8YG	54	250–2000	29/29
858	(TG)8RT	52	330–1845	21/21
859	(TG)8RC	55	300–1340	25/26
860	(TG)8RA	52	330–2400	17/18
889	DBD(AC)7	52	320–1800	26/27
890	VHV(GT)7	54	310–1575	36/37
891	HVH(TG)7	54	340–1495	27/27
868	(GAA)6	50	470–1675	30/30
873	(GACA)4	50	595–1355	10/13
				570/584

^*Single letter abbreviations for mixed-base positions: Y = (C, T), R = (A, G), B = (non A), D = (non C), V = (non T), H = (non G).

^†Determined empirically.

^‡Number of polymorphic fragments/number of fragments amplified.