Fig. 3.

Cell proliferation arrest with EBV genome destruction. (A) Cell proliferation curves after different CRISPR treatments. Five independent sgEBV1–7 treatments are shown here. (B–D) Flow-cytometry scattering signals before (B), 5 d after (C), and 8 d after (D) sgEBV1–7 treatments. (E–G) Annexin V Alexa647 and DAPI staining results before (E), 5 d after (F), and 8 d after (G) sgEBV1–7 treatments. Blue and red correspond to subpopulation P3 and P4 in (B–D). (H and I) Microscopy revealed apoptotic cell morphology after sgEBV1–7 treatment. (J–M) Nuclear morphology before (J) and after (K–M) sgEBV1–7 treatment. (Scale bars: 10 μm.)