Fig. 3.
HuR associates with eIF2ak4 and influences translation factor phosphorylation in the developing neocortex. (A, Left) Western blot analysis of total neocortical lysates collected from WT and HuR-cKO at E13 and P0. (Right) Quantification is shown (n = 3). *P < 0.05 (t test normalized with respect to GAPDH loading control). (B) HuR was immunoprecipitated (IP) from E18 neocortical lysates and analyzed by Western blot for interaction with translation factor candidates. HuR was used as the positive control. (C) HuR and eIF2ak4 reverse coimmunoprecipitation from HuR-cKO and eIF2ak4 kinase-domain mutant (eIF2ak4mut). (D) Immunohistochemistry for HuR (red) and eIF2ak4 (green) in E13 and E16 neocortical coronal sections showed their colocalization in cytoplasmic puncta (white arrows) of VZ RG and differentiating CP neurons. DAPI is shown in blue.