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. 2014 Aug 25;111(36):E3815–E3824. doi: 10.1073/pnas.1408305111

Fig. 4.

Fig. 4.

HuR and eIF2ak4 regulate specificity of translation factors and ribosomal proteins in neocortical polysomes. (A) Schematic of our model for how HuR and eIF2ak4 may interact in polysomes to influence mRNA translation. (B, Left) Western blot analysis of P0 neocortical lysates (n = 3 cortices) from WT and HuR-cKO shows unchanged total levels of translation factors and ribosomal proteins. (Right) Western blot analysis of P0 neocortical density-gradient fractionations shows that eIF2ak4mut disrupts HuR polysome enrichment, and both HuR-cKO and eIF2ak4mut disrupt eIF2ak4 polysome enrichment. The levels of eIF2a remain stable. (C) Western blot analysis (Left) and quantification (Right) of P0 WT, HuR-cKO, and eIF2ak4mut neocortices to measure the association of eIF5, eEF1A1, Rpl5, and Rpl7 with 40S-60S-80S and polysomal fractions.