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Amount of 5-[3H]-dCTP formed after incubation of NbNrdD with 5-[3H]-CTP, dATP, TrxA, TrxB, and limiting amounts of NADPH at 30 °C for 3 h. Stoichiometry of dCTP produced per NADPH added is 0.97. The concentration of NbNrdD G• in the reaction is ∼1 μM, and the ∼26 turnovers per G• that occur without addition of NADPH are attributed to the reduction of TrxA by residual DTT carried over from the NbNrdD storage buffer (∼27 μM).
