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. 2014 Aug 25;111(36):13034–13039. doi: 10.1073/pnas.1413987111

Fig. 3.

Fig. 3.

Fluorescence of Arch variants measured in E. coli resuspended in phosphate buffer at pH 5, pH 7, and pH 9. All listed mutations are in the Arch(DETC) background. Individual mutations are written in black text (e.g., T27A refers to the Arch variant containing the mutations T27A, D95E, and T99C). Mutations of recombined variants from the initial error-prone PCR mutagenesis library are written in red text (e.g., V59A/P196S refers to the Arch variant containing the mutations V59A, D95E, T99C, and P196S). Arch-5 contains the mutations V59A, P60L, D95E, T99C, and P196S. Arch-7 contains the mutations V59A, P60L, D95E, T99C, P196S, D222S, and A225C.