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. 2014 Aug 25;111(36):13063–13068. doi: 10.1073/pnas.1404166111

Fig. 3.

Fig. 3.

EM model and SAXS models of ΔN-chordin and the CHRD region. A 3D structure of ΔN-chordin was generated using TEM. (A) Selected class sum images (Upper) and reprojections (Lower) that represent different views of ΔN-chordin are shown (box size = 27 nm). Class sum images were used to generate a 3D TEM model (B). (C) The experimentally determined hydrodynamic properties from AUC are compared with values predicted from the TEM model for ∆N-chordin using HYDROMIC (32) and the SAXS model for the CHRD region using HYDROPRO (33). (D) MALLS profile of the CHRD region showing refractive index and molecular mass against elution volume, predicting Mw of 55.5 kDa. (E) Velocity AUC analysis of the CHRD region showing a peak corresponding to a monomeric species. (F) Ab initio model generated from SAXS data using DAMMIN shown in three orthogonal views.