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. 2014 Nov 11;9(1):471–477. doi: 10.3892/ol.2014.2701

Figure 3.

Figure 3

miR-27a inhibits cell proliferation by reducing the expression of KRAS in ESCC cell lines and animal models. (A) TE-11, (B) ECA-109 and (C) TE-1 cells were transfected with miR-27a or miRNA-NC. Following reseeding, cell numbers were measured using an MTS assay kit at 0, 24, 48 and 72 h. (D) KRAS expression was detected by reverse transcription-quantitative polymerase chain reaction following siK-ras transfection into the TE-1 cell line or siNC as a control. (E) TE-1 cells were transfected with siK-ras or siNC, using the same treatment as the miR-27a mimic transfection. (F) Western blots demonstrating the expression of K-ras following miR-27a mimic or or miRNA-NC transfection. (G) Western blots demonstrating the expression of K-ras following siK-ras or siNC transfection. GAPDH served as the internal control. (H) Nude mice were photographed 24 days after injection with TE-1 cells transfected with pEGFP-miR-27 or pEGFP as a control. (I) Tumor size was measured every four days and tumor growth curves were generated. Each assay was performed in triplicate.*P<0.05, **P<0.01; ***P<0.001. miRNA, microRNA; KRAS, Kirsten rat sarcoma viral oncogene homolog; ESCC, esophagus squamous cell carcinoma; NC, negative control; siK-ras, small interfering K-ras; siNC, siK-ras negative control.