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. 2014 Nov 11;9(1):471–477. doi: 10.3892/ol.2014.2701

Table I.

Primer sequences for the construction of the recombinant expression vectors used in the present study.

Plasmid Primer sequence Restriction enzyme
pGL3-Kras-3′-UTR Forward: GAGCAAAGATGGTAAAAAGA XbaI
Reverse: TAAATATAGCCCCAAAATGG EcoRV
pGL3-Kras-3′-UTR/mut Forward: AACTAGCAATGCGTCTCATAAAGAAACTGAATACCTAAGATTTCTGTC
Reverse: GACAGAAATCTTAGGTATTCAGTTTCTTTATGAGACGCATTGCTAGTT
pcDNA3.1-Kras Forward: ATGACTGAATATAAACTTGTGGTAG XhoI
Reverse: ACTAGATAAAACACAGAATAGGGAT EcoRV
pcDNA3.1-Kras/mut Forward: AACTAGCAATGCGTCTCATAAAGAAACTGAATACCTAAGATTTCTGTC
Reverse: GACAGAAATCTTAGGTATTCAGTTTCTTTATGAGACGCATTGCTAGTT
pEGFP-miR-27a Forward: AAGTTGCTGTAGCCTCCTTGTCC XbaI
Reverse: CCCACTCACCCACCTATCTATGC EcoRI

UTR, untranslated region.