Table I.
Plasmid | Primer sequence | Restriction enzyme |
---|---|---|
pGL3-Kras-3′-UTR | Forward: GAGCAAAGATGGTAAAAAGA | XbaI |
Reverse: TAAATATAGCCCCAAAATGG | EcoRV | |
pGL3-Kras-3′-UTR/mut | Forward: AACTAGCAATGCGTCTCATAAAGAAACTGAATACCTAAGATTTCTGTC | |
Reverse: GACAGAAATCTTAGGTATTCAGTTTCTTTATGAGACGCATTGCTAGTT | ||
pcDNA3.1-Kras | Forward: ATGACTGAATATAAACTTGTGGTAG | XhoI |
Reverse: ACTAGATAAAACACAGAATAGGGAT | EcoRV | |
pcDNA3.1-Kras/mut | Forward: AACTAGCAATGCGTCTCATAAAGAAACTGAATACCTAAGATTTCTGTC | |
Reverse: GACAGAAATCTTAGGTATTCAGTTTCTTTATGAGACGCATTGCTAGTT | ||
pEGFP-miR-27a | Forward: AAGTTGCTGTAGCCTCCTTGTCC | XbaI |
Reverse: CCCACTCACCCACCTATCTATGC | EcoRI |
UTR, untranslated region.