Figure 3.

Serum H₂S level and tumor gene expression in sulfur-treated mice. (A) Sulfur inhibited the expression of AR, PSA and NKX3.1. Total RNA was prepared from tumors and the expression of AR, PSA and NKX3.1 was measured by quantitative polymerase chain reaction. ^**P<0.01 and ^***P<0.001. (B) H₂S levels in mice serum. Mouse blood was collected from the heart and serum was isolated. H₂S levels were then measured by ELISA. ^*P<0.05. H₂S, hydrogen sulfide; AR, androgen receptor; PSA, prostate-specific antigen.