Fig. 3.

Effects of anoxia on the levels of PdSUS1 and PdSUS2 transcripts. RNA was extracted from turion segments incubated in air or under anoxia at 25 °C in the dark for 0 d, 1 d and 3 d. The amount of transcript was determined by RT–PCR. The level of 26S rRNA transcript was used as an internal control. A typical example in three independent experiments is shown.