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. 2005 Jul 18;96(4):717–726. doi: 10.1093/aob/mci223

Fig. 2.

Fig. 2.

Expression of E. coli PPase in roots of transgenic potato plants (‘Desiree’). (A) Schematic structure of the construct used for transformation of potato plants. STLS, leaf-stem-specific promoter; 35S, 35S CaMV promoter; Ω, TMV-U1 translational enhancer; T, octopine synthase terminator. (B) Northern blot analysis of roots of E. coli PPase-expressing plants (UPPa II-2) as well as of wild-type plants (Desiree WT) under aerated conditions, C, and after 4 d of hypoxic treatment, H, with two replicates each. (C) Enzymatic activity of soluble PPase in potato root extracts after 4 d of hypoxia, H, compared with aerated conditions, C. Activity values are means of at least 8–12 samples ± s.e. from four independent experiments. Asterisks show significant changes between aeration and hypoxia (*) and between wild-type and transgenic plants (**) at the 5 % level (t-test).