Table 1.
Fragment size (bp) |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Restriction enzyme |
No. |
Reference |
Mutant |
Chlorotypes |
|||||
HhaI | 1 | 5600 + X | → | 5680 | S3–S4–S7 | ||||
2 | 5300 + X | → | 5450 | S3–S4–S5–S6–S7–S8 | |||||
3 | 5300 | → | 5150 + X | S8 | |||||
a | 4760 | → | 4650 | S5–S6 | |||||
4 | 4080 + X | → | 3880 | S5–S6 | |||||
5 | 1545 + X | → | 1560 | S3–S4–S7 | |||||
6 | 7730 | → | 5210 + 2520 | S7 | |||||
DraI | a | 2680 | → | 2570 | S5–S6 | ||||
7 | 2045 + X | → | 2065 | S4 | |||||
b | 2040 | → | 1980 | S5–S6–S8 | |||||
c | 1550 | → | 1580 | S5–S6–S8 | |||||
AvaI | 8 | 10590 + 3440 | → | 14010 | S5–S6 | ||||
c | 5220 | → | 5250 | S5–S6 | |||||
9 | 3930 + X | → | 3900 | S5–S6 | |||||
10 | 3120 | → | 3050 + X | S8 | |||||
b | 2220 | → | 2180 | S5–S6 | |||||
BamHI | 11 | 9310 + X | → | 9390 | S6 | ||||
12 | 4520 + X | → | 4480 | S2 | |||||
13 | 2960 + X | → | 2970 | S5–S6 | |||||
a | 2950 | → | 2840 | S5–S6 |
Cork oak material was scored for RFLP cpDNA variation by using four restriction enzymes.
Changes attributable to site and length mutations are indicated with numbers and letters, respectively. Changes attributable to the same mutation are indexed with the same letter.
Some fragments could not be accurately sized due to small size or multiple superimposed bands (X).