Table 2.

Restriction fragment length changes (kb) compared with the fragment size observed in the Q. suber individuals possessing chlorotype A (Reference) in cork oak material and their occurrence in the other chlorotypes of ‘suber’ cpDNA lineage

Primers	Enzyme	Mutation no.	Reference		Fragment size mutant	Chlorotypes
trnC-trnD	TaqI	1	1100	→	1050	E
		2	1100	→	1000	B
		3	540	→	555	D, E
		4	335	→	340	C, D
trnS-trnR	HinfI	5	215	→	240	E
trnT-trnF	HinfI	6	290	→	280	D, E
		7	120	→	125	B
trnH-trnK	TaqI	8	70 + 40	→	110	D, E
trnF-trnV	TaqI	9	940 + 520	→	1460	D
		10	320	→	310	B

Cork oak material was scored for PCR–RFLP cpDNA variation using five combinations of primers/restriction enzymes.