Fig. 4.
Effect of three herbicides on the production and cross-linking of polysaccharide-bound hydroxycinnamic acids. Aliquots of 2-d-old maize culture were pre-treated with the herbicides for 2 h, then fed [14C]cinnamate for 3 h, after which the cells were assayed for polysaccharide-esterified phenolics by TLC in either (A) benzene/acetic acid or (B) toluene/acetic acid/water, and detected by autoradiography. The herbicide doses were (final concentrations in nm): isoxaben—a, 0; b, 360; c, 1200; d, 3600; e, 12000; f, 36000; oxaziclomefone—g, 0; h, 11; i, 33; j, 109; k, 330; l, 1090; diclofop methyl—m, 0; n, 117; o, 350; p, 1170; q, 3500; r, 11700. The positions of authentic markers are indicated (Cinn, cinnamate; Cou, p-coumarate; Fer, ferulate; Sin, sinapate; Caff, caffeate; DFA, 5,5′-diferulate); those in italics were not detectable in radioactive form. sf, Solvent front; ori, origin.