Fig. 8.
Effect of an OAC analogue (RPA800298) on mixed-linkage β-glucan (MLG) and starch biosynthesis. Maize cell cultures were treated with 0–162 nm of RPA800298 for 3 h and then with [14C]glucose for a further 3 h. The radiolabelled AIR was heated, then digested with licheninase and the oligosaccharides produced were paper chromatographed (A). Identities of peaks of radioactivity: G4G3G, the major MLG-derived trisaccharide; M2–M4, maltose to maltotetraose. No free [14C]glucose (Glc) was detectable. The MLG-derived tetrasaccharide, G4G4G3G, remained very near the origin (RF = 0), not resolved from undigested polysaccharides. The partially purified G4G3G was eluted from the appropriate strips of chromatography paper in (A) and re-run by electrophoresis in borate buffer (B). In this system, the M3 and M4 approximately co-migrate but are well resolved from G4G3G.