Table 1.
Description of primers used in RT-PCR for gene expression analyses
| Gene | Accession no | Primers 5′ → 3′ | PCR product (bp) |
|---|---|---|---|
| GAPDH | NM_001082253.1 | F: caa cga att tgg cta cag ca R: aaa ctg tga aga ggg gca ga |
186 |
| Sox-2 | NM_003106 | F: ttacctcttcct ccc act cca R: ggtagtgctgggacatgtgaa |
132 |
| Rex-1 | NM_174900 | F: aaaggttttcgaagcaagctc R: ctgcgagctgtt tag gat ctg |
185 |
| Nanog3 | NM_024865 | F: ctgtgatttgtgggcctgaa R: tgtttgcctttgggactggt |
153 |
| Nestin | NM_006617 | F: tccaggaacggaaaatcaag R: gcctcctcatcccct act tc |
120 |
| P53 | NM_001126112.2 | F: ccc agc caa aga aga aac ca R: gtt cca agg cct cat tca gct |
101 |
| pRB | NM_000321 | F: cag acc cag aag cca ttg aa R: ctg ggt gct cag aca gaa gg |
115 |
| GADD45 | NM_052850 | F: cca aga tgc cac aga tga ttg R: act cct tgg gtc cac ctg gta |
140 |
| CyclinD1 | NM_053056 | F: aga cct tcg ttg ccc tct gt R: cag tcc ggg tca cac ttg at |
181 |
Abbreviations: bp, base pairs; PCR, polymerase chain reaction; RT-PCR, real-time polymerase chain reaction.