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. 2014 Sep 30;15(1):828. doi: 10.1186/1471-2164-15-828

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© Logan et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Genome coverage profiles for three non-FMDV panel of viruses. Coverage of 10,000 was achieved for the majority of the EMCV-1 genome (olive). Peaks in coverage can be observed at the location of sequence specific primers used in the RT reaction (~4000 bp and ~8000 bp). A dip in coverage was evident at the poly(C) tract. The ERAV-1 genome showed between 10x and 100x coverage with visible peaks in coverage at the specific primer sites (~4000 bp and ~8000 bp) (black). Approximately 100x coverage of the majority of the VESV-B34 genome was achieved (blue).