Table 3.
Selected Blood and Bronchoalveolar Lavage Assays for MSC-treated and Placebo-treated Asthmatic Cats
| Assay | Txa | Baseline | Day 3 | Day 75 | Month 8 | Month 12 |
|---|---|---|---|---|---|---|
| BALFb eosinophil % | PBSc | 33 ± 14 | 45 ± 27 | 55 ± 8 | 40 ± 6 | 44 ± 8 |
| MSCd | 37 ± 11 | 64 ± 14 | 50 ± 22 | 41 ± 19 | 57 ± 12 | |
| Composite Clinical score | PBS | 3.3 ± 0.5 | 4.1 ± 0.9 | 4.3 ± 0.9 | 4.3 ± 0.9 | 4.3 ± 1.3 |
| MSC | 4.0 ± 0.5 | 3.4 ± 0.8 | 3.7 ± 1.3 | 3.2 ± 1.7 | 3.2 ± 1.2 | |
| VASe | PBS | 4.5 ± 2.2 | 7.4 ± 0.6 | 6.3 ± 1.4 | 5.2 ± 1.1 | 4.3 ± 2.2 |
| MSC | 5.9 ± 1.3 | 5.2 ± 2.0 | 5.2 ± 3.1 | 3.8 ± 3.3 | 2.8 ± 1.8 | |
| EC150RAWf | PBS | 1.3 (1.2-30.2) | 1.3 (1.2-1.9) | 1.7 (1.2-3.1) | 1.2 (0.3-1.9) | 3.2 (2.2-4.4) |
| MSC | 2.3 (1.2-12.9) | 1.3 (1.3-17.7) | 1.3 (1.2-13.7) | 1.3(1.1-49.5) | 4.0 (1.8-6.2) | |
| BALF IgE (EUg) | PBS | 258 (144-609) | 725 (242-1645) | 331 (121-706) | 997 (304-1776) | 220 (125-535) |
| MSC | 315 (123-1181) | 745 (103-1023) | 468 (105-1424) | 288 (146-1006) | 261 (79-2139) | |
| Serum IgE (EU) | PBS | 93 (17-603) | 448 (196-12271) | 587 (125-803) | 120 (19-2301) | 173 (24-11422) |
| MSC | 15 (14-256) | 16 (14-743) | 242 (19-3583) | 17 (15-2716) | 143 (17-19694) | |
| WBh IL-10 restimulation (pg/ml) | PBS | 406 (345-770) | 377 (177-680) | 125 (125-125) | 125 (125-125) | 134 (125-197) |
| MSC | 125 (125-136)* | 357 (158-865) | 125 (125-141) | 125 (125-125) | 221 (125-258) | |
| BALFi IL-10 restimulation (pg/ml) | PBS | 492 (158-828) | 635 (252-3197) | 405 (125-2423) | 646 (125-1917) | 546 (179-887) |
| MSC | 1306 (535-2204) | 864 (487-1569) | 569 (482-763) | 391 (149-936) | 824 (462-1368) | |
| BGAj-specific lymphocyte proliferation % | PBS | 3.9 ± 3.8 | 1.8 ± 2.6 | 1.2 ± 0.3 | 1.4 ± 1.2 | 0.7 ± 0.9 |
| MSC | 3.4 ± 3.1 | 1.5 ± 1.2 | 1.8 ± 1.6 | 1.3 ± 1.0 | 0.4 ± 0.5 |
Parametric data are expressed as mean ± standard deviation and non-parametric data are expressed at median (interquartile range).
*
p<0.05 in MSC-treated cats compared with placebo-treated cats.
a
Tx – treatment group
b
BALF – bronchoalveolar lavage fluid
c
PBS – placebo-treated asthmatic cats
d
MSC – MSC-treated asthmatic cats
e
VAS – visual analog scale of respiratory symptoms
f
EC150RAW – dose of BGA increasing baseline airway resistance by 150%
g
EU – ELISA units
h
WB – whole blood
i
BALF – bronchoalveolar lavage fluid
j
BGA – Bermuda grass allergen