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. 2014 Nov 4;6(11):3117–3128. doi: 10.3390/toxins6113117

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Protocol for the supplementation of dietary feed with mycotoxin adsorbent (MA) and for the sampling of urine and feed samples. Sterigmatocystin concentrations in the urine and feed samples collected at each sampling point were analyzed using liquid chromatography-tandem mass spectrometry. Herd 1: Zearalenone (ZEN)-contaminated herd that had shown persistently higher mean ZEN concentrations in the urine. Herd 2: No ZEN contamination. Both herds were fed from the same lot of concentrate, but they received different rice straw. *MA1: MA was mixed with dietary concentrate. *MA2: MA was topdressed on the dietary concentrate. **MA1: Typical straw was replaced by a new lot of straw with less contamination. **MA2: Twice the recommended volume of MA was topdressed on the dietary concentrate. Control: No MA was provided during the experimental period.