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. Author manuscript; available in PMC: 2014 Nov 28.
Published in final edited form as: Nat Commun. 2014 Apr 22;5:3674. doi: 10.1038/ncomms4674

Figure 2. MacQ based sensors exhibit transient but not steady state photocurrents.

Figure 2

(a) MacQ constructs generate a transient excitatory photocurrent at the onset of illumination, illustrated here by the inward current (top) evoked by illumination (blue bars) of a neuron expressing MacQ-mOrange2. The steady state photocurrent deviates negligibly from the baseline measured in the absence of illumination. An expanded view of the photocurrent transient (bottom) reveals its time course at the onset of illumination.

(b) Steady-state (ss) and transient (tr) photocurrents of MacQ-mOrange2 (n = 14 cells) and MacQ-mCitrine (n = 9 cells) in response to 530 nm and 505 nm illumination, respectively.

Illumination intensity at the specimen was 15 mW mm−2 for all panels. Error bars are s.e.m.