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Number of tartrate-resistant acid phosphatase (TRACP)-positive cells among RAW 264.7 cells induced by receptor-activated nuclear κB ligand (RANKL). RAW 264.7 cells were cultured with α-MEM containing RANKL (40 ng/ml) for 3, 5 and 7 days. TRAP-positive cells were stained using a TRACP staining kit. TRACP-positive cells were counted using Simple PCI software.
