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. 2014 Nov 4;9(1):49–54. doi: 10.3892/etm.2014.2050

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Expression of 5-HT receptor subtypes determined by western blot analysis in the sling and clasp fibers of the LES and circular muscle strips of the esophagus and stomach. (A) Bands of five 5-HT receptor subtypes in E, S, C and G were identified by western blotting. (B) IOD values of the bands. There were significant differences between the 5-HT subtypes in the same muscle strip (P<0.05), but no significant differences were identified in a single subtype between the four types of muscle strip (P>0.05). 5-HT, serotonin; LES, lower esophageal sphincter; IOD, integrated optical density; E, circular muscle strips of esophagus; S, sling fibers; C, clasp fibers; G, stomach.