Figure 2.

PAR-2 mRNA expression in SW1990 cells by RT-PCR detection. The cells were treated with VKGILS (50 μM), SLIGKV (50 μM) or trypsin (10 nM) for 18 h, or were not treated (medium only). RT-PCR was performed. β-actin served as the internal control. (A) A representative image is shown. (B) Data from at least three repeated RT-PCR experiments. ^**P<0.01 and ^*P<0.05 vs. the control group. RT-PCR, reverse transcription-polymerase chain reaction; VKGILS, Val-Lys-Gly-Ile-Leu-Ser; SLIGKV, Ser-Leu-Ile-Gly-Lys-Val; PAR-2, protease-activated receptor 2.