Figure 3.

Effect of VKGILS, trypsin and SLIGKV on SW1990 cell proliferation. The SW1990 cells were treated with trypsin, SLIGKV and VKGILS at the concentrations as indicated. The cells treated with medium were used as the control. MTT assay was performed. MTT solution (20μl; 5 g/l) was added to each well after 48 h of culture. Each well was then supplemented with 150 μl dimethyl sulfoxide and oscillated for 30 min at room temperature for the crystals to dissolve. The absorbance (A) value for each well at 490 nm wavelength was determined. This experiment was repeated three times. VKGILS, Val-Lys-Gly-Ile-Leu-Ser; SLIGKV, Ser-Leu-Ile-Gly-Lys-Val.