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. 2014 Nov 5;9(1):239–244. doi: 10.3892/etm.2014.2052

Figure 5.

Figure 5

Effect of VKGILS, trypsin and SLIGKV on MMP-2 and MMP-9 expression. The cells were treated with VKGILS-NH2 (50 μM), SLIGKV (50 μM) or trypsin (10 nM) for 24 h, or were not treated (medium only). (A) Reverse transcription-polymerase chain reaction detection. (B) Activity measurements of MMP-2 and MMP-9 enzymes by gelatin zymography detection. **P<0.01 and *P<0.05 vs. the control group. VKGILS, Val-Lys-Gly-Ile-Leu-Ser; SLIGKV, Ser-Leu-Ile-Gly-Lys-Val; MMP, matrix metalloproteinase; OD, optical density.