Figure 6.
Inhibition of Rac1 by Rac1-siRNA attenuates high glucose-induced hyperpermeability in RRECs. RRECs with or without transfection of Rac1-siRNA were treated with 25 mm glucose for 12 h. The [3H]sucrose permeability assay in RRECs was measured as counts per minute (C.P.M.). Each point represents the mean ± standard deviation of three independent experiments, each performed in triplicate (n=6). *P<0.05 vs. high glucose-induced RRECs without Rac1-siRNA transfection. Rac1, Ras-related C3 botulinum toxin substrate 1; RREC, rat retinal endothelial cell; siRNA, small interfering RNA; HG, high glucose.