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. 2014 Oct 16;4:987–995. doi: 10.1016/j.fob.2014.10.007

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© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Supplementary Fig. S1 — Binding isotherms obtained from fluorimetric titration of MTR with human recombinant histones H2A (A), H2B (B) H4 (C) at 25 °C. The insets show the emission spectra of 2 μM MTR in 10 mM Tris–HCl, pH 7.0 containing 150 mM NaCl in presence of increasing concentration of the histones. λ_ex = 470 nm. (D) Emission spectra of 2 μM MTR in presence of increasing concentrations of linker histone H1. Inset shows the variation of fluorescence intensity of MTR at emission maximum with increasing concentration of H1.