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Fluorimetric titration of MTR with histone dimer/tetramer. Emission spectra of 2 μM MTR in 10 mM Tris–HCl, pH 7.0 containing 150 mM NaCl at 25 °C in presence of H2A-H2B dimer (A) and H3–H4 tetramer (B). λex = 470 nm. Panels (C), (D) show the corresponding fitted isotherms obtained by non-linear curve fitting analysis.
